REAL-TIME PCR

qPCR
Master Mix

Formula Total = per reaction × (reactions + extra wells)

1

Reaction

Assay type

SYBR TaqMan Probe

Product preset Reactions

Reaction volume

µL

Plate format

48 well 96 well 384 well

Extra wells

0 10 20 30

2

Per reaction

2X Master mix

µL

Forward primer

µL

Reverse primer

µL

Template

µL

RESULT

Preparation

Check the inputs.