PTA
Western Blot
PharmTech Archive
Concentration
SDS-PAGE
Western Blot
Gel Recipe
Formula
30% Acrylamide/Bis stock × target gel %
1
Gel setup
Gel count
gels
Acrylamide/Bis stock
30%, 29:1
30%, 37.5:1
Resolving gel
Gel percent
6%
8%
10%
12%
15%
Custom
Custom percent
%
Volume
mL/gel
Stacking gel
Gel percent
3%
4%
5%
Custom
Custom percent
%
Volume
mL/gel
Extra volume
0%
10%
20%
30%
Calculate
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RESULT
Recipe
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